De Neve M, De Loose M, Jacobs A, Van Houdt H, Kaluza B, Weidle U, Van Montagu M, Depicker A
Laboratorium voor Genetica, Universiteit Gent, Belgium.
Transgenic Res. 1993 Jul;2(4):227-37. doi: 10.1007/BF01977353.
The yield and assembly of an IgG1 antibody and its derived F(ab) fragment were compared in Nicotiana and Arabidopsis. The results obtained showed a lot of interclonal variability. For 45% of the primary transgenic calluses, antigen-binding entities represented less than 0.1% of the total soluble protein (TSP). Only two of the 103 analysed transformants contained more than 1% of antigen-binding protein, with 1.26% being the highest yield. Analogous amounts of complete antibody and F(ab) accumulated in primary callus tissue. Moreover, yields were in the same range for both species as far as primary callus tissue is concerned. However, the accumulation of the F(ab) fragment in leaf tissue of regenerated plants differed significantly between Nicotiana and Arabidopsis. The F(ab) fragment accumulated to only 0.044% of TSP in Nicotiana leaves but up to 1.3% in Arabidopsis leaves. Furthermore, both species showed differences in the assembly pattern of the complete antibody. Whereas Arabidopsis contained primarily fully assembled antibodies of 150 kDa, Nicotiana showed an abundance of fragments in the 50 kDa range.
在烟草和拟南芥中比较了IgG1抗体及其衍生的F(ab)片段的产量和组装情况。所得结果显示出大量的克隆间变异性。对于45%的初级转基因愈伤组织，抗原结合实体占总可溶性蛋白（TSP）的比例不到0.1%。在103个分析的转化体中，只有两个含有超过1%的抗原结合蛋白，最高产量为1.26%。在初级愈伤组织中积累的完整抗体和F(ab)的量相当。此外，就初级愈伤组织而言，两个物种的产量处于相同范围。然而，再生植物叶片组织中F(ab)片段的积累在烟草和拟南芥之间存在显著差异。F(ab)片段在烟草叶片中仅积累到TSP的0.044%，但在拟南芥叶片中高达1.3%。此外，两个物种在完整抗体的组装模式上也存在差异。拟南芥主要含有150 kDa的完全组装抗体，而烟草在50 kDa范围内有大量片段。
