一种可靠的[14C]海藻糖合成方法。
A dependable method for the synthesis of [14C]trehalose.
作者信息
Stambuk B U, Crowe J H, Crowe L M, Panek A D, de Araujo P S
机构信息
Departamento de Bioquímica, Universidade de S. Paulo, Brazil.
出版信息
Anal Biochem. 1993 Jul;212(1):150-3. doi: 10.1006/abio.1993.1305.
A new method for the preparation of [14C]trehalose was developed, based on the ability of yeast cells to accumulate trehalose under stress. The method is simple and reliable. It utilizes a yeast strain in which the gene that encodes for phosphoglucoisomerase has been deleted. Thus, exogenously supplied glucose is not metabolized, but is instead converted to trehalose. The [14C]-trehalose obtained is pure, it is hydrolyzed by trehalase, and it is not susceptible to the action of alpha-glucosidase. The yield of this method is in the order of 35% of the [14C]glucose supplied.
基于酵母细胞在应激状态下积累海藻糖的能力，开发了一种制备[14C]海藻糖的新方法。该方法简单可靠。它利用了一种编码磷酸葡萄糖异构酶的基因已被删除的酵母菌株。因此，外源提供的葡萄糖不会被代谢，而是转化为海藻糖。所获得的[14C]海藻糖是纯的，它能被海藻糖酶水解，并且不易受α-葡萄糖苷酶的作用影响。该方法的产率约为所提供[14C]葡萄糖的35%。
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