Pawłowski P, Szutowicz I, Marszałek P, Fikus M
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw.
Biophys J. 1993 Jul;65(1):541-9. doi: 10.1016/S0006-3495(93)81056-7.
Recently proposed analysis of the extensil stress developed in a cellular membrane subjected to an alternating electric field (Pawłowski, P., and M. Fikus, 1993. Bioelectrorheological model of the cell. 4. Analysis of the extensil deformation of the membrane in an alternating field. Biophys. J. 65:535-540) was applied in calculations of extensil stress threshold values, sigma eo[d], producing experimentally observed electrodestruction of cells within the frequency range of 7 x 10(1) - 3 x 10(5) Hz. It was shown that the susceptibility (s[d] = 1/sigma eo[d]), of the membrane to this process varies with field frequency and depends on the type of cells. Electrodestruction is facilitated in the 10(5)-Hz field. A rheological hypothesis explaining the experimentally observed dependence of membrane stability on electric field frequency was proposed and successfully tested for two other phenomena: electroporation and electrofusion.
最近提出的对置于交变电场中的细胞膜产生的拉伸应力的分析方法（Pawłowski, P. 和 M. Fikus, 1993年。细胞的生物电流变学模型。4. 交变电场中膜的拉伸变形分析。《生物物理学杂志》65:535 - 540）被用于计算拉伸应力阈值σeo[d]，该阈值可在7×10¹ - 3×10⁵Hz频率范围内产生实验观察到的细胞电破坏现象。结果表明，膜对该过程的敏感性（s[d] = 1/σeo[d]）随场频率变化，且取决于细胞类型。在10⁵Hz的场中电破坏更容易发生。提出了一个流变学假设，解释了实验观察到的膜稳定性对电场频率的依赖性，并成功地对另外两种现象进行了验证：电穿孔和电融合。
