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一种18千道尔顿布鲁氏菌细胞质蛋白的特性，该蛋白似乎是人类和牛布鲁氏菌病活动性感染的血清学标志物。

Characterization of an 18-kilodalton Brucella cytoplasmic protein which appears to be a serological marker of active infection of both human and bovine brucellosis.

作者信息

Goldbaum F A, Leoni J, Wallach J C, Fossati C A

机构信息

Sección Brucelosis, Hospital F. J. Muñíz, Buenos Aires, Argentina.

出版信息

J Clin Microbiol. 1993 Aug;31(8):2141-5. doi: 10.1128/jcm.31.8.2141-2145.1993.

DOI:10.1128/jcm.31.8.2141-2145.1993
PMID:8370742
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC265711/
Abstract

Some anticytoplasmic protein monoclonal antibodies (MAbs) from mice immunized by infection with Brucella ovis cells have been obtained. One of these MAbs, BI24, was used to purify by immunoaffinity a protein with a pI of 5.6 and a molecular mass of 18 kDa. This protein was present in all of the rough and smooth Brucella species studied, but it could not be detected in Yersinia enterocolitica 09. Three internal peptides of this protein were partially sequenced; no homology with other bacterial proteins was found. The immunogenicity of the 18-kDa protein was studied with both human and bovine sera by a capture enzyme-linked immunosorbent assay system with MAb BI24.

摘要

已获得一些由感染绵羊布鲁氏菌细胞免疫的小鼠产生的抗细胞质蛋白单克隆抗体（MAb）。其中一种单克隆抗体BI24用于通过免疫亲和法纯化一种等电点为5.6、分子量为18 kDa的蛋白质。该蛋白质存在于所有研究的粗糙型和光滑型布鲁氏菌物种中，但在小肠结肠炎耶尔森菌09中未检测到。对该蛋白质的三个内部肽段进行了部分测序；未发现与其他细菌蛋白有同源性。通过使用单克隆抗体BI24的捕获酶联免疫吸附测定系统，用人血清和牛血清研究了18 kDa蛋白质的免疫原性。

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1
Characterization of an 18-kilodalton Brucella cytoplasmic protein which appears to be a serological marker of active infection of both human and bovine brucellosis.一种18千道尔顿布鲁氏菌细胞质蛋白的特性，该蛋白似乎是人类和牛布鲁氏菌病活动性感染的血清学标志物。
J Clin Microbiol. 1993 Aug;31(8):2141-5. doi: 10.1128/jcm.31.8.2141-2145.1993.
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Evolution of vitamin B2 biosynthesis: 6,7-dimethyl-8-ribityllumazine synthases of Brucella.维生素B2生物合成的进化：布鲁氏菌的6,7-二甲基-8-核糖基卢马嗪合酶
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A microplate method of enzyme-linked immunosorbent assay and its application to malaria.一种酶联免疫吸附测定的微孔板方法及其在疟疾中的应用。
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Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.从电转印到聚偏二氟乙烯膜上的皮摩尔量蛋白质中获得的序列。
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Specific antibodies detected during relapse of human brucellosis.
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