Sugita O, Okada M, Kimura S, Matsuto T
Department of Laboratory Medicine, Niigata University School of Medicine.
Rinsho Byori. 1993 Aug;41(8):914-8.
The present work details an improved application of kit to the measurement of lipid peroxides in human serum. Kits based on the reaction of lipid hydroperoxides with a leucomethylene blue derivative in the presence of hemoglobin. Overall sensitivity was increased by taking samples 5 times larger than those indicated in product literature for the system. It was necessary, however, to compensate actual data for protein error. To do this, the following empirically obtained formula was employed: A = a/(-3.45x + 98) x 100, where A (nmol/ml) indicates compensated data for lipid peroxides, a (nmol/ml) indicates the data for the actual measurement of lipid peroxides, and x (g/dl) indicates the total protein. We obtained what we considered to be satisfactory results by the above formula, as it allowed us to observe that lipid peroxides in fresh human serum undergo a change, momentarily.
本研究详细介绍了一种试剂盒在测定人血清中脂质过氧化物方面的改进应用。该试剂盒基于脂质氢过氧化物在血红蛋白存在下与无色亚甲蓝衍生物的反应。通过采集比产品说明书中该系统指示样本量多5倍的样本，总体灵敏度得以提高。然而，有必要对蛋白质误差的实际数据进行补偿。为此，采用了以下通过实验得出的公式：A = a/(-3.45x + 98) × 100，其中A（nmol/ml）表示脂质过氧化物的补偿数据，a（nmol/ml）表示脂质过氧化物实际测量的数据，x（g/dl）表示总蛋白。通过上述公式，我们获得了我们认为满意的结果，因为它使我们能够观察到新鲜人血清中的脂质过氧化物会瞬间发生变化。
