Ohishi N, Ohkawa H, Miike A, Tatano T, Yagi K
Biochem Int. 1985 Feb;10(2):205-11.
To determine the absolute amount of lipid hydroperoxides in biological materials, a simple and sensitive colorimetric method was newly developed, based on the reaction of lipid hydroperoxides with a leucomethylene blue derivative in the presence of hemoglobin. The amount of methylene blue formed was measured by its absorbance at 666 nm to calculate the amount of lipid hydroperoxides using cumene hydroperoxide as external standard. By this method, lipid hydroperoxide concentrations of less than 7.5 nmol/tube were accurately determined.
为了测定生物材料中脂质氢过氧化物的绝对含量，基于脂质氢过氧化物在血红蛋白存在下与无色亚甲蓝衍生物的反应，新开发了一种简单且灵敏的比色法。通过在666nm处测量生成的亚甲蓝的吸光度来测定其含量，以异丙苯过氧化氢作为外标计算脂质氢过氧化物的含量。通过这种方法，可以准确测定低于7.5nmol/管的脂质氢过氧化物浓度。
