Tateishi T, Yoshimine N, Kuzuya F
Exp Gerontol. 1987;22(2):103-11. doi: 10.1016/0531-5565(87)90045-3.
Serum lipid peroxide (LPO) in 72 healthy male subjects was studied using a newly developed assay method based on the reaction of LPO with methylene blue derivative (MCDP; 10-N-Methylcarbamoyl-3,7-dimethylamino-10 H-phenothiazine). This method is specific for lipid hydroperoxide and the relation is equimolar. The value obtained was 7.63 +/- 2.78 nmol/ml. Positive correlations were observed between LPO and height (r = 0.290), body weight (r = 0.244) and hemoglobin (r = 0.248). However, no significant correlation was observed between serum LPO level and other clinical data, including age. In addition, serum LPO value measured by this method did not show significant correlation with that measured by the thiobarbituric acid method in rat serum. This discrepancy might be due to the differences in substrates measured by each method.
采用一种新开发的基于脂质过氧化物（LPO）与亚甲蓝衍生物（MCDP；10-N-甲基氨基甲酰基-3,7-二甲基氨基-10H-吩噻嗪）反应的检测方法，对72名健康男性受试者的血清脂质过氧化物（LPO）进行了研究。该方法对脂质氢过氧化物具有特异性，且关系为等摩尔。得到的值为7.63±2.78 nmol/ml。观察到LPO与身高（r = 0.290）、体重（r = 0.244）和血红蛋白（r = 0.248）之间呈正相关。然而，血清LPO水平与包括年龄在内的其他临床数据之间未观察到显著相关性。此外，用该方法测得的大鼠血清LPO值与用硫代巴比妥酸法测得的值之间未显示出显著相关性。这种差异可能是由于每种方法所测量的底物不同所致。
