Pappas A A, Thompson J R, Porter W H, Gadsden R H
University of Arkansas for Medical Sciences, Little Rock 72205-7199.
J Anal Toxicol. 1993 Jul-Aug;17(4):230-2. doi: 10.1093/jat/17.4.230.
A precise, accurate, and nondestructive method for the detection and quantitation of serum ethanol in humans using proton (1H) nuclear magnetic resonance spectroscopy (MRS) was developed. The 1H MRS method was linear within the range of 30-1500 mg/L. The lowest detectable ethanol concentration was 15 mg/L, with 30 mg/L being the lowest level reproducibly quantitated. Within-run and day-to-day coefficients of variation (CV) ranged from 0.6 to 2.7% and 0.5 to 3.5%, respectively. The excellent day-to-day CVs indicate a negligible loss of ethanol due to volatilization during analysis. Fifteen human serum samples found to be negative for ethanol by headspace gas chromatography (HSGC) had no ethanol as detected by 1H MRS. Twenty-eight human serum samples with ethanol concentrations (determined by HSGC) ranging from 370 to 4440 mg/L were accurately reproduced by 1H MRS. The 1H MRS method required no pretreatment and was nondestructive, thereby allowing for further analysis by confirmatory methods.
开发了一种使用质子（1H）核磁共振波谱法（MRS）检测和定量人体血清乙醇的精确、准确且无损的方法。1H MRS方法在30 - 1500 mg/L范围内呈线性。最低可检测乙醇浓度为15 mg/L，最低可重复性定量水平为30 mg/L。批内和日间变异系数（CV）分别为0.6%至2.7%和0.5%至3.5%。出色的日间CV表明分析过程中乙醇因挥发造成的损失可忽略不计。通过顶空气相色谱法（HSGC）检测为乙醇阴性的15份人体血清样本，1H MRS检测未发现乙醇。1H MRS准确再现了28份乙醇浓度（由HSGC测定）在370至4440 mg/L之间的人体血清样本。1H MRS方法无需预处理且无损，从而可通过确证方法进行进一步分析。
