Institute of Forensic Medicine, Medical Center-University of Freiburg, Faculty of Medicine, University of Freiburg, 79104 Freiburg, Germany.
Division of Medical Physics, Department of Diagnostic and Interventional Radiology, Medical Center-University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany.
Int J Mol Sci. 2023 Aug 31;24(17):13499. doi: 10.3390/ijms241713499.
In many parts of the world, ethanol is a widely consumed substance that displays its effect in the brain, the target organ for desired, but also negative impact. In a previous study, the ethanol concentrations were analyzed in different regions of the brain by magnetic resonance spectroscopy (MRS). In this study, the same method is used to demonstrate the kinetics of the ethanol concentration in the human brain after oral ethanol uptake. A drinking study was performed with 10 healthy participants. After the uptake of ethanol in a calculated amount leading to a plasma ethanol concentration of 0.92 g/L (19.95 mM corresponding to a blood ethanol concentration of 0.7 g/kg), brain ethanol concentrations were continuously measured by means of MRS on a 3 Tesla human magnetic resonance imaging (MRI) system. For the data acquisition a single-voxel sLASER sequence was used, with the volume of interest located in the occipital cortex. Intermittently, blood samples were taken and plasma was analyzed for ethanol using headspace gas chromatography with flame ionization detection (HS-GC-FID). The obtained MRS brain ethanol curves showed distinct inter-individual differences; however, a good intra-individual correlation of plasma and brain ethanol concentrations was observed. The results suggest a rapid equilibration between blood and brain. The ethanol concentrations measured in the brain were substantially lower than the measured plasma ethanol results, suggesting an MRS visibility of about 63% for ethanol in brain tissue. The maximum individual ethanol concentrations in the brain (normalized to water content) ranged between 7.1 and 14.1 mM across the cohort, while the highest measured plasma concentrations were in the range between 0.35 g/L (9.41 mM) and 0.95 g/L (20.52 mM).
在世界上许多地区，乙醇是一种广泛消费的物质，它在大脑中发挥作用，既是目标器官，也是负面作用的靶点。在之前的一项研究中，通过磁共振波谱（MRS）分析了大脑不同区域的乙醇浓度。在这项研究中，同样的方法被用来证明口服乙醇摄入后大脑中乙醇浓度的动力学。对 10 名健康参与者进行了一项饮酒研究。在计算出的摄取量下，使血浆乙醇浓度达到 0.92g/L（对应于血液乙醇浓度为 0.7g/kg 的 19.95mM）后，通过 3 Tesla 人体磁共振成像（MRI）系统的 MRS 连续测量脑内乙醇浓度。对于数据采集，使用单体 sLASER 序列，感兴趣体积位于枕叶皮层。间歇性地采集血样，并使用顶空气相色谱-火焰离子化检测（HS-GC-FID）分析血浆中的乙醇。获得的 MRS 脑乙醇曲线显示出明显的个体间差异；然而，观察到血浆和脑乙醇浓度的个体内相关性良好。结果表明血液和大脑之间快速平衡。大脑中测量的乙醇浓度明显低于测量的血浆乙醇结果，表明大脑组织中乙醇的 MRS 可见度约为 63%。大脑中个体最大乙醇浓度（归一化为水含量）在队列中范围在 7.1 到 14.1mM 之间，而最高测量的血浆浓度范围在 0.35g/L（9.41mM）和 0.95g/L（20.52mM）之间。
