子宫腺肌病组织中雌激素合成的证据。
Evidence for estrogen synthesis in adenomyotic tissues.
作者信息
Yamamoto T, Noguchi T, Tamura T, Kitawaki J, Okada H
机构信息
Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Japan.
出版信息
Am J Obstet Gynecol. 1993 Sep;169(3):734-8. doi: 10.1016/0002-9378(93)90654-2.
OBJECTIVE
To investigate steroidogenesis in eutopic and ectopic endometrial tissues in adenomyosis.
STUDY DESIGN
Aromatase and estrone sulfatase activities were determined by anion-exchange resin column chromatography, thin-layer chromatography, and cocrystallization. The effects of danazol on the activity of these enzymes were also examined. Moreover, localization of aromatase in eutopic and ectopic endometrial tissues was immunohistochemically examined with antihuman placental aromatase cytochrome P-450 antibody.
RESULTS
Aromatase and estrone sulfatase activities were detected in ectopic endometrium. The activity of these enzymes was significantly suppressed by danazol. In addition, aromatase was immunohistochemically detected in glandular cells of eutopic and ectopic endometrial tissues.
CONCLUSIONS
The results suggest that estrogen is synthesized in the eutopic and ectopic endometrial tissues of women with uterine adenomyosis and that it may affect the growth of adenomyosis. Danazol suppressed synthesis of estrogen in vitro.
目的
研究子宫腺肌病在位及异位子宫内膜组织中的类固醇生成。
研究设计
通过阴离子交换树脂柱色谱法、薄层色谱法和共结晶法测定芳香化酶和雌酮硫酸酯酶活性。还检测了达那唑对这些酶活性的影响。此外，使用抗人胎盘芳香化酶细胞色素P-450抗体通过免疫组织化学方法检测在位和异位子宫内膜组织中芳香化酶的定位。
结果
在异位子宫内膜中检测到芳香化酶和雌酮硫酸酯酶活性。这些酶的活性被达那唑显著抑制。此外，通过免疫组织化学方法在在位和异位子宫内膜组织的腺细胞中检测到芳香化酶。
结论
结果表明，子宫腺肌病患者的在位和异位子宫内膜组织中合成雌激素，且其可能影响子宫腺肌病的生长。达那唑在体外抑制雌激素的合成。
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