Breitholtz-Emanuelsson A, Olsen M, Oskarsson A, Palminger I, Hult K
Royal Institute of Technology, Department of Biochemistry and Biotechnology, Stockholm, Sweden.
J AOAC Int. 1993 Jul-Aug;76(4):842-6.
A method for determining ochratoxin A in milk has been elaborated in which the sample was subjected to a liquid-liquid extraction step and then purified on a silica gel column packed in a Pasteur pipet. The purified samples were analyzed by ion-pair liquid chromatography with fluorescence detection. The detection and quantitation limits for determination of ochratoxin A in cow's milk were 10 and 40 ng ochratoxin A/L milk, respectively. The same limits were valid for the analysis of human milk. A total of 36 cow's milk and 40 human milk samples were analyzed. All samples were collected in Sweden. Ochratoxin A was found in 5 (14%) of the cow's milk samples (range 10-40 ng/mL) and in 23 (58%) of the human milk samples (range 10-40 ng/L). Blood samples were collected from the mothers who gave milk samples. A total of 39 samples were analyzed. All blood samples contained ochratoxin A in concentrations exceeding the quantitation limit (60 ng/L blood). The mean concentration of ochratoxin A in the samples was 167 ng/L blood (range 90-940 ng/L). The concentration of ochratoxin A in human milk was < or = 0.1 of that in the human blood.
已制定了一种测定牛奶中赭曲霉毒素A的方法，其中样品经过液液萃取步骤，然后在填充于巴氏滴管中的硅胶柱上进行纯化。纯化后的样品通过离子对液相色谱法和荧光检测进行分析。牛奶中赭曲霉毒素A测定的检测限和定量限分别为10 ng赭曲霉毒素A/L牛奶和40 ng赭曲霉毒素A/L牛奶。同样的限值适用于人乳分析。共分析了36份牛奶样品和40份人乳样品。所有样品均在瑞典采集。在5份（14%）牛奶样品中发现了赭曲霉毒素A（范围为10 - 40 ng/mL），在23份（58%）人乳样品中发现了赭曲霉毒素A（范围为10 - 40 ng/L）。从提供母乳样品的母亲那里采集了血液样品。共分析了39份样品。所有血液样品中赭曲霉毒素A的浓度均超过定量限（60 ng/L血液）。样品中赭曲霉毒素A的平均浓度为167 ng/L血液（范围为90 - 940 ng/L）。人乳中赭曲霉毒素A的浓度小于或等于人血中浓度的0.1。
