Wiesner A
Institut für Zoologie, Freie Universität Berlin, Germany.
Dev Comp Immunol. 1993 Jul-Aug;17(4):291-300. doi: 10.1016/0145-305x(93)90001-7.
Humoral immune responses of Galleria mellonella larvae were provoked by transfer of hemolymph lysate supernatant preparations (HLS) from untreated donor larvae to recipient larvae. Eighteen hours after transfer the antibacterial activity in cell-free hemolymph of recipient larvae was as high as after injection of high dosages of living Enterobacter cloacae beta 12 bacteria. Filtration of HLS (FHLS; 0.2 microns filter) prior to injection enhanced its induction capacity. Injection of FHLS concentrates (> 30 < 100 kDa) provoked the same immune responses as injection of FHLS itself. Heat treatment did not destroy the induction capacity of FHLS. Hemolymph samples of larvae immunized by hemolymph transfer or by injection of bacteria showed the same characteristic protein pattern in SDS-PAGE. At least six new or enhanced bands were detected in hemolymph of immunized larvae. These bands were missing or very weak in hemolymph from untreated or saline injected larvae.
将未处理供体幼虫的血淋巴裂解物上清液制剂（HLS）转移至受体幼虫，可激发大蜡螟幼虫的体液免疫反应。转移18小时后，受体幼虫无细胞血淋巴中的抗菌活性与注射高剂量活阴沟肠杆菌β12细菌后的活性一样高。注射前对HLS进行过滤（FHLS；0.2微米滤器）可增强其诱导能力。注射FHLS浓缩物（>30<100 kDa）引发的免疫反应与注射FHLS本身相同。热处理并未破坏FHLS的诱导能力。通过血淋巴转移或注射细菌免疫的幼虫的血淋巴样本在SDS-PAGE中显示出相同的特征性蛋白质图谱。在免疫幼虫的血淋巴中检测到至少六条新的或增强的条带。这些条带在未处理或注射生理盐水的幼虫的血淋巴中缺失或非常微弱。
