Anderson B, Kelly C, Threlkel R, Edwards K
Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333.
J Infect Dis. 1993 Oct;168(4):1034-6. doi: 10.1093/infdis/168.4.1034.
One of the diagnostic criteria for cat-scratch disease is a positive delayed-type hypersensitivity skin test reaction to an antigen prepared from purulent material aspirated from a cat-scratch disease-involved lymph node. Polymerase chain reaction coupled with DNA sequence analysis was used to identify organisms found in these skin test antigen preparations that may be responsible for eliciting this response. Two independent sources of cat-scratch disease skin test antigens yielded DNA sequences characteristic of the newly described rickettsia, Rochalimaea henselae. These results coupled with previous serologic data strongly suggest that R. henselae plays a central role in the etiology of cat-scratch disease.
猫抓病的诊断标准之一是对从猫抓病相关淋巴结吸出的脓性物质制备的抗原进行延迟型超敏皮肤试验反应呈阳性。聚合酶链反应结合DNA序列分析用于鉴定这些皮肤试验抗原制剂中发现的可能引发这种反应的微生物。猫抓病皮肤试验抗原的两个独立来源产生了新描述的立克次体——汉赛巴尔通体的特征性DNA序列。这些结果与先前的血清学数据相结合，强烈表明汉赛巴尔通体在猫抓病的病因学中起核心作用。
