Yagi M, Wada K, Sakata M, Kokubo M, Haga M
Faculty of Pharmaceutical Sciences, Higashi Nippon Gakuen University, Hokkaido, Japan.
Yakugaku Zasshi. 1993 Aug;113(8):596-9. doi: 10.1248/yakushi1947.113.8_596.
The serum 4-O-methylpyridoxine (MPN) levels in a 21 months-old patient with gin-nan food poisoning were determined by HPLC. The blood of the patient was taken at 8.5 and 15.5 h after taking about 50 ginkgo albumens. After deproteinization of the serum, the supernatant was pretreated with Sep-pak C18 cartridge and was applied to HPLC. HPLC was performed with a Hibar LiChrosorb RP-18 (4.0 mm i.d. x 250 mm, 7 microns) using a fluorescence detector (wave length of excitation and fluorescence; 290 and 400 nm, respectively). The serum MPN level was determined by the absolute calibration method. The determination limit of MPN in the serum was 0.05 micrograms/ml. The serum MPN concentration was 0.09 micrograms/ml at 8.5 h after taking ginkgo seeds, and was less than the determination limit of MPN (0.05 micrograms/ml) at 15.5 h. MPN, isolated from the seed of Ginkgo biloba L., is responsible for "gin-nan food poisoning," and its cardinal symptoms are mainly tonic and/or clonic convulsions and loss of consciousness. Infants are particularly vulnerable. This method may be available for proving the gin-nan food poisoning, chemically.
采用高效液相色谱法（HPLC）测定了一名21个月大的白果食物中毒患者血清中4 - O - 甲基吡哆醇（MPN）的含量。该患者在食用约50克银杏果仁后8.5小时和15.5小时采集血液。血清经去蛋白处理后，上清液用Sep - pak C18柱预处理，然后进行HPLC分析。HPLC采用Hibar LiChrosorb RP - 18柱（内径4.0 mm×250 mm，7微米），配备荧光检测器（激发波长和发射波长分别为290纳米和400纳米）。血清MPN含量采用绝对校准法测定。血清中MPN的测定限为0.05微克/毫升。食用银杏果仁后8.5小时血清MPN浓度为0.09微克/毫升，15.5小时时低于MPN测定限（0.05微克/毫升）。从银杏种子中分离出的MPN是导致“白果食物中毒”的原因，其主要症状主要为强直性和/或阵挛性惊厥以及意识丧失。婴儿尤其易受影响。该方法可从化学角度用于证实白果食物中毒。
