Reid G, Servin A L, Bruce A W, Busscher H J
Department of Microbiology and Immunology, University of Western Ontario, London, Canada.
Microbios. 1993;75(302):57-65.
Three strains of urogenital lactobacilli were found to adhere in phosphate buffered saline to human uroepithelial cells in vitro according to thermodynamic principles, and to adhere in culture medium to intestinal cells with no such correlation. The most hydrophilic strain (water contact angle 54 degrees) L. casei RC-17 was the most adherent to uroepithelial (118 bacteria per cell) and intestinal cells (165 bacteria per cell). A direct correlation was found between bacterial hydrophilicity and adhesion to uroepithelial cells for the three strains tested. An extracellular adhesin, which appeared to be proteinaceous, and a trypsin-insensitive cell wall adhesin were identified. It was evident that high levels of bacterial retention could be reproduced in vitro, perhaps indicating the potential colonizing capacity of these strains in vivo.
根据热力学原理，发现三株泌尿生殖系统乳酸杆菌在体外磷酸盐缓冲盐水中能黏附于人尿道上皮细胞，而在培养基中能黏附于肠道细胞，但不存在这种相关性。亲水性最强的菌株（水接触角为54度）干酪乳杆菌RC-17对尿道上皮细胞（每个细胞黏附118个细菌）和肠道细胞（每个细胞黏附165个细菌）的黏附性最强。在所测试的三株菌株中，发现细菌亲水性与对尿道上皮细胞的黏附之间存在直接相关性。鉴定出一种似乎是蛋白质性质的细胞外黏附素和一种对胰蛋白酶不敏感的细胞壁黏附素。很明显，在体外可以重现高水平的细菌滞留，这可能表明这些菌株在体内具有潜在的定殖能力。
