Griffiths R, Tiwari B
Agriculture and Food Research Council Unit of Ecology and Behaviour, Department of Zoology, Oxford, United Kingdom.
Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8324-6. doi: 10.1073/pnas.90.18.8324.
It is difficult to identify sex in many species of eukaryotic organism. This can considerably impede research into their biology. Fortunately, one sex often possesses a unique chromosome termed Y or W. When DNA markers are available for these chromosomes, then sex identification becomes straightforward. We describe a technique that facilitates the isolation of such markers. The procedure makes use of low-stringency PCR to screen randomly selected primers for their ability to amplify sex-specific loci.
