Dawson D A, Wilke T S
Department of Animal Science, College of Veterinary Medicine, University of Tennessee, Knoxville 37901-1071.
Ecotoxicol Environ Saf. 1991 Apr;21(2):215-26. doi: 10.1016/0147-6513(91)90023-i.
The joint toxic action of three binary mixtures was determined for the embryo malformation endpoint of the aquatic FETAX (frog embryo teratogenesis assay: Xenopus) test system. Osteolathyrogenic compounds and short-chain carboxylic acids, representing separate, distinct modes of action for induction of malformation, were selected for testing in 96-hr, static-renewal tests. Three mixtures were tested for each combination, with each combination being tested on three separate occasions. Using toxic unit analysis, the combination of osteolathyrogens and the combination of carboxylic acids produced strictly additive (concentration addition) rates of malformation, while the combination of an osteolathyrogen and a carboxylic acid was less-than-additive (response addition) for induction of malformation. Therefore, developmental malformation may have value as an endpoint in mixture toxicity hazard assessment.
针对水生蛙胚胎致畸试验（FETAX，即非洲爪蟾胚胎致畸试验）系统的胚胎畸形终点，测定了三种二元混合物的联合毒性作用。选择骨软化剂化合物和短链羧酸，它们代表诱导畸形的不同、独特作用模式，用于96小时静态更新试验。每种组合测试三种混合物，每种组合在三个不同场合进行测试。使用毒性单位分析，骨软化剂组合和羧酸组合产生的畸形率严格呈相加性（浓度相加），而骨软化剂与羧酸组合在诱导畸形方面则低于相加性（反应相加）。因此，发育畸形可作为混合物毒性危害评估的一个终点。
