Schenkman R P, Vandekerckhove F, Schenkman S
Department of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, São Paulo, Brazil.
Infect Immun. 1993 Mar;61(3):898-902. doi: 10.1128/iai.61.3.898-902.1993.
We have used a Chinese hamster ovary cell mutant (Lec2) that express much less sialic acid on the surface than the parental cell line (Pro5) to investigate whether sialic acid plays a role during cell invasion by Trypanosoma cruzi. Trypomastigotes derived from a tissue culture (corresponding to bloodstream trypomastigotes) and metacyclic trypomastigotes (corresponding to infective stages of the insect vector) invaded the Lec2 mutant less efficiently than the parental cell line. Invasion of the Lec2 mutant cells could be restored to the Pro5 level by resialylation of the mutant cells with T. cruzi trans-sialidase and sialyllactose. Conversely, pretreatment of the Pro5 parental cells with bacterial neuraminidase decreased invasion. These results indicate that sialic acid associated with the host cell contributes to invasion by T. cruzi.
我们使用了一种中国仓鼠卵巢细胞突变体（Lec2），其表面唾液酸的表达量远低于亲代细胞系（Pro5），以研究唾液酸在克氏锥虫细胞侵袭过程中是否发挥作用。源自组织培养的锥鞭毛体（对应于血液中的锥鞭毛体）和循环后期锥鞭毛体（对应于昆虫媒介的感染阶段）对Lec2突变体的侵袭效率低于亲代细胞系。用克氏锥虫转唾液酸酶和唾液乳糖对突变体细胞进行再唾液酸化处理后，Lec2突变体细胞的侵袭能力可恢复到Pro5水平。相反，用细菌神经氨酸酶预处理Pro5亲代细胞会降低侵袭能力。这些结果表明，与宿主细胞相关的唾液酸有助于克氏锥虫的侵袭。
