Pauza C D, Kornbluth R, Emau P, Richman D D, Deftos L J
Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison 53706.
J Leukoc Biol. 1993 Feb;53(2):157-64. doi: 10.1002/jlb.53.2.157.
We studied the effects of vitamin D3 compounds on the replication of human immunodeficiency virus type 1 (HIV-1) in the monoblastoid cell line U937 and in primary monocyte-derived macrophage cultures to understand how modulators of monocyte/macrophage effector function might affect the pathogenesis of HIV-1 infection. U937 cell cultures exposed to 1, alpha 25-dihydroxyvitamin D3 prior to HIV-1 infection showed enhanced virus replication that was apparently due to increased cellular resistance to viral cytopathic effects; a marked inhibition of virus replication was noted in cells exposed to 1 alpha,25-dihydroxyvitamin D3 subsequent to infection. Exposure of blood-derived monocyte/macrophages to vitamin D3 compounds prior to infection also affected virus growth; in most cases, substantial inhibition of HIV-1 replication was noted in vitamin D3-treated macrophage cultures. Our results demonstrate that vitamin D3 compounds with recognized abilities to induce cellular differentiation can modulate HIV-1 infection of human macrophages.
我们研究了维生素D3化合物对1型人类免疫缺陷病毒（HIV-1）在单核细胞样细胞系U937以及原代单核细胞衍生的巨噬细胞培养物中复制的影响，以了解单核细胞/巨噬细胞效应功能的调节剂如何影响HIV-1感染的发病机制。在HIV-1感染前暴露于1,α25-二羟基维生素D3的U937细胞培养物显示病毒复制增强，这显然是由于细胞对病毒细胞病变效应的抗性增加；在感染后暴露于1α,25-二羟基维生素D3的细胞中，病毒复制受到显著抑制。在感染前将血液来源的单核细胞/巨噬细胞暴露于维生素D3化合物也会影响病毒生长；在大多数情况下，在维生素D3处理的巨噬细胞培养物中，HIV-1复制受到显著抑制。我们的结果表明，具有公认的诱导细胞分化能力的维生素D3化合物可以调节人类巨噬细胞的HIV-1感染。
