通过聚合酶链反应对人三叉神经节中潜伏性水痘-带状疱疹病毒DNA进行定量分析。
Quantitation of latent varicella-zoster virus DNA in human trigeminal ganglia by polymerase chain reaction.
作者信息
Mahalingam R, Wellish M, Lederer D, Forghani B, Cohrs R, Gilden D
机构信息
Department of Neurology, University of Colorado School of Medicine, Denver 80262.
出版信息
J Virol. 1993 Apr;67(4):2381-4. doi: 10.1128/JVI.67.4.2381-2384.1993.
Competitive polymerase chain reaction was used to quantitate latent varicella-zoster virus (VZV) DNA in human trigeminal ganglia. Ganglionic DNA from five subjects was amplified with oligonucleotide primers specific for VZV gene 28. Two of the samples were also analyzed with primers specific for VZV gene 62. Our results indicated that there are 6 to 31 copies of the VZV genome in every 100,000 ganglionic cells.
采用竞争性聚合酶链反应对人三叉神经节中潜伏的水痘-带状疱疹病毒（VZV）DNA进行定量分析。用针对VZV基因28的寡核苷酸引物对5名受试者的神经节DNA进行扩增。其中两个样本还用针对VZV基因62的引物进行了分析。我们的结果表明，每100,000个神经节细胞中有6至31个VZV基因组拷贝。
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