Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, D208 Veterinary Medical Center, 48824-1314 East Lansing, MI, USA.
BMC Vet Res. 2013 Sep 22;9:185. doi: 10.1186/1746-6148-9-185.
Herpes simplex virus 1 (HSV-1) and varicella zoster virus (VZV) cause extensive intra-ocular and neural infections in humans and are closely related to Felid herpes virus 1 (FeHV-1). We report the extent of intra-ocular replication and the extent and morphological aspects of neural replication during the acute and latent phases of FeHV-1 infection. Juvenile, SPF cats were inoculated with FeHV-1. Additional cats were used as negative controls. Cats were euthanized on days 6, 10, and 30 post-inoculation.
FeHV-1 was isolated from the conjunctiva, cornea, uveal tract, retina, optic nerve, ciliary ganglion (CG), pterygopalatine ganglion (PTPG), trigeminal ganglion (TG), brainstem, visual cortex, cerebellum, and olfactory bulb of infected cats during the acute phase, but not the cranial cervical ganglion (CCG) and optic chiasm. Viral DNA was detected in all tissues during acute infection by a real-time quantitative PCR assay. On day 30, viral DNA was detected in all TG, all CCG, and 2 PTPG. Histologically mild inflammation and ganglion cell loss were noted within the TG during acute, but not latent infection. Using linear regression, a strong correlation existed between clinical score and day 30 viral DNA copy number within the TG.
The correlation between clinical score and day 30 viral DNA copy number suggests the severity of the acute clinical infection is related to the quantity of latent viral DNA. The histologic response was similar to that seen during HSV-1 or VZV infection. To the author's knowledge this is the first report of FeHV-1 infection involving intraocular structures and autonomic ganglia.
单纯疱疹病毒 1 型（HSV-1）和水痘带状疱疹病毒（VZV）可导致人类眼部和神经的广泛感染，与猫疱疹病毒 1 型（FeHV-1）密切相关。我们报告了 FeHV-1 感染的急性期和潜伏期内眼部和神经的复制程度以及神经复制的程度和形态学特征。将幼 SPF 猫接种 FeHV-1。另外一些猫作为阴性对照。感染后第 6、10 和 30 天处死猫。
在急性期，从感染猫的结膜、角膜、葡萄膜、视网膜、视神经、睫状神经节（CG）、翼腭神经节（PTPG）、三叉神经节（TG）、脑干、视皮层、小脑和嗅球中分离到 FeHV-1，但未从颅颈神经节（CCG）和视交叉中分离到。通过实时定量 PCR 检测，在急性期所有组织中均检测到病毒 DNA。第 30 天，在所有 TG、所有 CCG 和 2 个 PTPG 中检测到病毒 DNA。在急性期而非潜伏感染时，TG 中可见轻度炎症和神经节细胞丢失。使用线性回归，TG 中第 30 天的临床评分与病毒 DNA 拷贝数之间存在很强的相关性。
临床评分与 TG 中第 30 天病毒 DNA 拷贝数之间的相关性表明急性临床感染的严重程度与潜伏病毒 DNA 的数量有关。组织学反应与 HSV-1 或 VZV 感染相似。据作者所知，这是首次报道 FeHV-1 感染涉及眼部结构和自主神经节。
