Efstathiou S, Minson A C, Field H J, Anderson J R, Wildy P
J Virol. 1986 Feb;57(2):446-55. doi: 10.1128/JVI.57.2.446-455.1986.
Herpes simplex virus-specific DNA sequences have been detected by Southern hybridization analysis in both central and peripheral nervous system tissues of latently infected mice. We have detected virus-specific sequences corresponding to the junction fragment but not the genomic termini, an observation first made by Rock and Fraser (Nature [London] 302:523-525, 1983). This "endless" herpes simplex virus DNA is both qualitatively and quantitatively stable in mouse neural tissue analyzed over a 4-month period. In addition, examination of DNA extracted from human trigeminal ganglia has shown herpes simplex virus DNA to be present in an "endless" form similar to that found in the mouse model system. Further restriction enzyme analysis of latently infected mouse brainstem and human trigeminal DNA has shown that this "endless" herpes simplex virus DNA is present in all four isomeric configurations.
通过Southern杂交分析，在潜伏感染小鼠的中枢神经系统和外周神经系统组织中均检测到单纯疱疹病毒特异性DNA序列。我们检测到了与连接片段相对应的病毒特异性序列，但未检测到基因组末端的序列，这一观察结果最早由Rock和Fraser（《自然》[伦敦]302:523 - 525, 1983年）做出。这种“无端”的单纯疱疹病毒DNA在长达4个月的分析期内在小鼠神经组织中质量和数量上均保持稳定。此外，对从人三叉神经节提取的DNA进行检测表明，单纯疱疹病毒DNA以一种与在小鼠模型系统中发现的类似的“无端”形式存在。对潜伏感染的小鼠脑干和人三叉神经DNA进行的进一步限制性内切酶分析表明，这种“无端”的单纯疱疹病毒DNA以所有四种异构体构型存在。
