Hyypiä T, Kallajoki M, Maaronen M, Stanway G, Kandolf R, Auvinen P, Kalimo H
Department of Virology, University of Turku, Finland.
Virus Res. 1993 Jan;27(1):71-8. doi: 10.1016/0168-1702(93)90113-2.
Coxsackieviruses are divided into A and B subgroups on the basis of their pathogenicity in newborn mice. Although used in the classification of these viruses, our understanding of the details of the infection is incomplete due to the lack of sensitive and specific techniques to localize the viruses in affected tissue. We have used in situ hybridization to detect coxsackievirus genomes in tissues of newborn mice after infection by five serotypes (A2, A9, A21, B3 and B4) through different administration routes. Our results indicate that coxsackie A viruses are able to affect both skeletal and heart muscle while the coxsackievirus B subgroup infects a wide range of tissues. In addition to striated muscle these include central nervous system, liver, exocrine pancreas and brown fat. This model will make it possible to analyze molecular factors determining tissue tropism.
柯萨奇病毒根据其对新生小鼠的致病性分为A、B两个亚组。尽管这一特性被用于这些病毒的分类，但由于缺乏在受感染组织中定位病毒的灵敏且特异的技术，我们对感染细节的了解并不完整。我们运用原位杂交技术，检测了新生小鼠在经五种血清型（A2、A9、A21、B3和B4）通过不同给药途径感染后的组织中的柯萨奇病毒基因组。我们的结果表明，柯萨奇A组病毒能够影响骨骼肌和心肌，而柯萨奇B组病毒感染广泛的组织。除横纹肌外，这些组织还包括中枢神经系统、肝脏、外分泌胰腺和棕色脂肪。该模型将使分析决定组织嗜性的分子因素成为可能。
