人肝癌细胞系PLC-PRF-5及其不同程度细胞分化的突变克隆的细胞遗传学分析。

Cytogenetic analysis of human hepatocarcinoma cell line PLC-PRF-5 and its mutant clones with different degrees of cell differentiation.

作者信息

Grabovskaya I L, Tugizov S M, Glukhova L A, Kushch A A

机构信息

D.I. Ivanovsky Institute of Virology of Russian Academy of Medical Sciences, Moscow.

出版信息

Cancer Genet Cytogenet. 1993 Feb;65(2):147-51. doi: 10.1016/0165-4608(93)90224-a.

DOI:10.1016/0165-4608(93)90224-a

PMID:8384075

Abstract

A detailed analysis was made of the karyotype of human hepatocarcinoma cell line PLC-PRF-5 containing an integrated hepatitis B viral genome and 10 mutant clones derived from the line. These clones are drug resistant and display features of cell differentiation. Cytogenetic manifestations of gene amplification common to many cells resistant to drugs were not observed in these clones, but certain tendencies of karyotype evolution involving material from chromosomes 7 and 11, as well as chromosomes 5 and 15, were recorded for a group of cytostatic-resistant clones. An increase in polyploid cell number and number of cells with chromosome pulverization as compared with the original line was noted in the clones. This phenomenon may be related to the cytopathic effect of the hepatitis B viral proteins, which have a higher expression in the clones.

摘要

对含有整合型乙型肝炎病毒基因组的人肝癌细胞系PLC-PRF-5及其10个衍生自该细胞系的突变克隆进行了详细的核型分析。这些克隆具有耐药性并表现出细胞分化特征。在这些克隆中未观察到许多耐药细胞常见的基因扩增的细胞遗传学表现，但记录到一组抗细胞生长抑制剂的克隆存在涉及7号和11号染色体以及5号和15号染色体物质的核型进化趋势。与原始细胞系相比，克隆中多倍体细胞数量和具有染色体粉碎的细胞数量增加。这种现象可能与乙型肝炎病毒蛋白的细胞病变效应有关，这些蛋白在克隆中表达较高。