Porter H J, Padfield C J, Peres L C, Hirschowitz L, Berry P J
Department of Paediatric Pathology, Bristol Royal Hospital for Sick Children.
J Clin Pathol. 1993 Feb;46(2):154-8. doi: 10.1136/jcp.46.2.154.
To examine the light and electron microscopic features of appendices removed at the time of surgical reduction of intussusception in children; and to confirm that the viral inclusions seen in some of them are due to adenovirus.
A series of 39 appendices from cases of intussusception and 15 control appendices were reviewed. Light microscopic examination of haematoxylin and eosin stained sections was performed on all of them and one appendix with large numbers of inclusions was examined by electron microscopy. Non-isotopic in situ hybridisation using a biotinylated DNA probe was carried out on sections of appendix from 30 of the cases of intussusception and from the 15 controls.
Light microscopic examination showed viral inclusions in 19 of the appendices from the cases of intussusception and in none of the controls. Electron microscopic examination showed viral particles with the typical features of adenovirus. Most of the appendices with viral inclusions in the haematoxylin and eosin stained sections also contained adenovirus DNA as shown by in situ hybridisation.
Viral inclusions seen in appendices from cases of intussusception are caused by adenovirus. Adenovirus DNA was not demonstrable in appendices from cases of intussusception without viral inclusions and the aetiological factors involved in intussusception in these children remain unknown.
研究儿童肠套叠手术复位时切除的阑尾的光镜和电镜特征；并证实其中一些阑尾中所见的病毒包涵体是由腺病毒引起的。
回顾了39例肠套叠病例的阑尾以及15例对照阑尾。对所有阑尾进行苏木精和伊红染色切片的光镜检查，并对1例有大量包涵体的阑尾进行电镜检查。使用生物素化DNA探针进行非同位素原位杂交，对30例肠套叠病例和15例对照的阑尾切片进行检测。
光镜检查显示，肠套叠病例的19例阑尾中有病毒包涵体，而对照阑尾中均未发现。电镜检查显示病毒颗粒具有腺病毒的典型特征。苏木精和伊红染色切片中有病毒包涵体的大多数阑尾经原位杂交显示也含有腺病毒DNA。
肠套叠病例阑尾中所见的病毒包涵体由腺病毒引起。在无病毒包涵体的肠套叠病例的阑尾中未检测到腺病毒DNA，这些儿童肠套叠的病因仍不清楚。
