Smyth M, Hall J, Fry E, Stuart D, Stanway G, Hyypiä T
Laboratory of Molecular Biophysics, Oxford, U.K.
J Mol Biol. 1993 Mar 20;230(2):667-9. doi: 10.1006/jmbi.1993.1179.
Coxsackievirus A9 has been crystallized as small rhombic dodecahedra of maximum dimension 0.3 mm. These crystals have been shown, using synchrotron radiation, to diffract X-rays to beyond 3 A, and to have a stability in the beam comparable to that of other related virus crystals. The unit cell is tetragonal with dimensions a = b = 495 A, c = 695 A and alpha = beta = gamma = 90 degrees, with a space group of P4n22. A substantial body of diffraction data has been collected and this crystal form appears to be suitable for structure determination. Phasing of these data will be attempted using molecular replacement.
柯萨奇病毒A9已结晶为最大尺寸为0.3毫米的小菱形十二面体。利用同步辐射已表明，这些晶体能将X射线衍射至3埃以上，并且在光束中的稳定性与其他相关病毒晶体相当。晶胞为四方晶系，尺寸a = b = 495埃，c = 695埃，α = β = γ = 90°，空间群为P4n22。已经收集了大量衍射数据，这种晶体形式似乎适合进行结构测定。将尝试使用分子置换法对这些数据进行相位分析。
