Smyth M, Fry E, Stuart D, Lyons C, Hoey E, Martin S J
Laboratory of Molecular Biophysics, Oxford, U.K.
J Mol Biol. 1993 Jun 5;231(3):930-2. doi: 10.1006/jmbi.1993.1339.
Bovine enterovirus (BEV) strain VG-5-27 derived from an infectious cDNA clone has been crystallized as extended hexagonal plates. Virus recovered from crystals produced cytopathic effect in BHK cells. These crystals diffract X-rays from high energy synchrotron sources to beyond 2.7 A. The crystal system is monoclinic, space group P2(1) with unit cell dimensions a = 388 A, b = 390 A, c = 360 A, beta = 113 degrees. The virion is 300 A in diameter and one whole particle constitutes the crystallographic asymmetric unit, giving rise to 60-fold non-crystallographic redundancy.
源自感染性 cDNA 克隆的牛肠道病毒（BEV）株 VG-5-27 已结晶为延长的六边形板状。从晶体中回收的病毒在 BHK 细胞中产生细胞病变效应。这些晶体能从高能同步辐射源衍射 X 射线，衍射极限超过 2.7 Å。晶体系统为单斜晶系，空间群为 P2(1)，晶胞参数 a = 388 Å，b = 390 Å，c = 360 Å，β = 113°。病毒粒子直径为 300 Å，一个完整的粒子构成晶体学不对称单元，产生 60 倍的非晶体学冗余。
