Toth K S, Zhang C X, Lipton H L, Luo M
Department of Microbiology, University of Alabama, Birmingham 35294.
J Mol Biol. 1993 Jun 20;231(4):1126-9. doi: 10.1006/jmbi.1993.1357.
Theiler's murine encephalomyelitis virus (TMEV) is a member of the picornavirus family. Mice infected with TMEV serve as models for the study of human neurological diseases including multiple sclerosis. Preparations of the GDVII strain of Theiler's virus have been crystallized using the hanging drop technique. When exposed to high intensity synchrotron radiation, these monoclinic crystals diffracted X-rays to at least 3.0 A resolution. The unit cell has a C-centered lattice with dimensions: a = 575.2 A, b = 324.0 A and c = 558.4 A, beta = 108.2 degrees. The molecular mass and cell dimensions imply that there is an entire virus particle per asymmetric unit, suggesting the presence of 60-fold non-crystallographic redundancy. This GDVII crystal form appears to be suitable for high-resolution structure determination.
泰勒氏鼠脑脊髓炎病毒（TMEV）是微小核糖核酸病毒科的成员。感染TMEV的小鼠可作为研究包括多发性硬化症在内的人类神经疾病的模型。泰勒氏病毒GDVII株的制剂已采用悬滴法结晶。当暴露于高强度同步加速器辐射时，这些单斜晶体将X射线衍射至至少3.0埃的分辨率。晶胞具有C心晶格，尺寸为：a = 575.2埃，b = 324.0埃，c = 558.4埃，β = 108.2度。分子量和晶胞尺寸表明每个不对称单元中有一个完整的病毒颗粒，这表明存在60倍的非晶体学冗余。这种GDVII晶体形式似乎适合用于高分辨率结构测定。
