Nikkels A F, Debrus S, Sadzot-Delvaux C, Piette J, Delvenne P, Rentier B, Piérard G E
Department of Dermatopathology, University of Liège, Belgium.
Virchows Arch A Pathol Anat Histopathol. 1993;422(2):121-6. doi: 10.1007/BF01607163.
Herpes simplex (HSV) and varicella-zoster (VZV) skin infections share so many histological similarities that distinguishing between them may prove to be impossible. We developed and characterized a new monoclonal antibody, VL8, IgG kappa isotype, directed to the VZV envelope glycoprotein gpI. Immunohistochemistry with VL8 appeared highly sensitive and specific on formalin-fixed paraffin-embedded biopsies and a clear-cut distinction between HSV and VZV infections was possible. The pattern of VL8 immunolabelling in VZV infections was strikingly different from that found in HSV infections studied with polyclonal antibodies to HSV I and II. Double immunolabelling revealed the VL8 positivity of sebaceous cells, endothelial cells, Mac 387- and CD68-positive monocyte-macrophages, and factor XIIIa-positive perivascular, perineural and interstitial dendrocytes. Intracytoplasmic VL8 labelling of endothelial cells and perivascular dendrocytes was found at the site of leukocytoclastic vasculitis.
单纯疱疹（HSV）和水痘带状疱疹（VZV）皮肤感染在组织学上有许多相似之处，以至于可能无法区分它们。我们开发并鉴定了一种新的单克隆抗体VL8，IgG κ同种型，针对VZV包膜糖蛋白gpI。在福尔马林固定石蜡包埋活检组织上，VL8免疫组化显示出高度敏感性和特异性，能够明确区分HSV和VZV感染。VZV感染中VL8免疫标记模式与用针对HSV I和II的多克隆抗体研究的HSV感染中发现的模式明显不同。双重免疫标记显示皮脂腺细胞、内皮细胞、Mac 387和CD68阳性单核巨噬细胞以及因子XIIIa阳性血管周围、神经周围和间质树突状细胞呈VL8阳性。在白细胞破碎性血管炎部位发现内皮细胞和血管周围树突状细胞有胞浆内VL8标记。
