Wang W, Napoli J L, Ballow M
Department of Pediatrics, Children's Hospital, Buffalo, NY 14222.
Clin Exp Immunol. 1993 Apr;92(1):164-8. doi: 10.1111/j.1365-2249.1993.tb05964.x.
In this study we examined the effects of retinol (ROH), a metabolic precursor of retinoic acid (RA), on Staphylococcus aureus Cowan I (SAC)-induced immunoglobulin synthesis of cord blood mononuclear cells (CBMC) and adult peripheral blood mononuclear cells (PBMC). ROH augmented SAC-induced IgM synthesis of CBMC by 5.9 +/- 1.5-fold (n = 7, mean +/- s.d.), and IgG synthesis of adult PBMC by 16.3 +/- 5.1-fold (n = 3) at optimal concentrations of 10(-6) M and 10(-11) M, respectively. No augmenting effects could be demonstrated for the other immunoglobulin isotypes. Time-course studies showed that the synthesis of IgM by CBMC was accelerated with detectable immunoglobulin in supernatant fluids starting on day 3. ROH augmented immunoglobulin synthesis of CBMC stimulated by Epstein-Barr virus (EBV), a T cell-independent polyclonal activator, and of EBV-transformed B cell clones (2.5 +/- 0.2 and 4.1 +/- 1.5-fold increase, respectively), which suggests that ROH can act directly on B cells to enhance immunoglobulin synthesis. In contrast, when ROH was preincubated with cord blood T cells, washed and added to the B cell-enriched fraction with SAC, no increase (0.9-1.8-fold) in IgM synthesis was obtained. Thus, the principal mechanism(s) by which ROH augments immunoglobulin synthesis is by acting on B cells. This is in contrast to the immunoglobulin-enhancing effects of RA which is mediated by T cells, or T cell products, e.g. cytokine. Our studies suggest that RA and ROH may have different pathways of immunoglobulin-enhancing effects, perhaps mediated by different retinoid binding proteins resulting in gene activation and immunoglobulin synthesis.
在本研究中，我们检测了视黄醇（ROH）（维甲酸（RA）的一种代谢前体）对金黄色葡萄球菌考恩I株（SAC）诱导的脐血单个核细胞（CBMC）和成人外周血单个核细胞（PBMC）免疫球蛋白合成的影响。在最佳浓度分别为10⁻⁶M和10⁻¹¹M时，ROH使SAC诱导的CBMC的IgM合成增加了5.9±1.5倍（n = 7，平均值±标准差），使成人PBMC的IgG合成增加了16.3±5.1倍（n = 3）。对于其他免疫球蛋白亚型，未显示出增强作用。时间进程研究表明，CBMC的IgM合成从第3天开始加速，上清液中可检测到免疫球蛋白。ROH增强了由爱泼斯坦-巴尔病毒（EBV）（一种不依赖T细胞的多克隆激活剂）刺激的CBMC以及EBV转化的B细胞克隆的免疫球蛋白合成（分别增加2.5±0.2倍和4.1±1.5倍），这表明ROH可直接作用于B细胞以增强免疫球蛋白合成。相反，当ROH与脐血T细胞预孵育、洗涤后加入富含B细胞的组分及SAC时，未获得IgM合成的增加（0.9 - 1.8倍）。因此，ROH增强免疫球蛋白合成的主要机制是作用于B细胞。这与RA的免疫球蛋白增强作用相反，RA的这种作用是由T细胞或T细胞产物（如细胞因子）介导的。我们的研究表明，RA和ROH可能具有不同的免疫球蛋白增强作用途径，也许是由不同的类视黄醇结合蛋白介导，从而导致基因激活和免疫球蛋白合成。
