Li C, Louise C J, Shi W, Adler J
Program in Cell and Molecular Biology, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706.
J Bacteriol. 1993 Apr;175(8):2229-35. doi: 10.1128/jb.175.8.2229-2235.1993.
Wild-type Escherichia coli was not motile when grown in tryptone broth under the following adverse conditions: the presence of high temperature [J. Adler and B. Templeton, J. Gen. Microbiol. 46:175-184, 1967; R. B. Morrison and J. McCapra, Nature (London) 192:774-776, 1961; K. Ogiuti, Jpn. J. Exp. Med. 14:19-28, 1936], high concentrations of salts, high concentrations of carbohydrates, high concentrations of low-molecular-weight alcohols, or the pressure of gyrase inhibitors. Under all these conditions, growth was necessary for the loss of motility. This loss of motility was correlated with a reduction in the amount of cellular flagellin. We isolated and studied mutants that are resistant to suppression of motility by some of these conditions, because of the ability to synthesize flagella under these conditions. The mutations were mapped to 42 min, a region of the chromosome where many of the flagellar genes map. We also studied the effect of a preexisting gyrA mutation which allowed flagellar formation in the presence of nalidixate.
存在高温[J. 阿德勒和B. 邓普顿，《普通微生物学杂志》46:175 - 184，1967；R. B. 莫里森和J. 麦卡普拉，《自然》（伦敦）192:774 - 776，1961；K. 荻田，《日本实验医学杂志》14:19 - 28，1936]、高浓度盐、高浓度碳水化合物、高浓度低分子量醇或回旋酶抑制剂的压力。在所有这些条件下，生长对于运动性丧失是必要的。这种运动性丧失与细胞鞭毛蛋白量的减少相关。我们分离并研究了一些突变体，这些突变体由于在这些条件下能够合成鞭毛，因而对其中一些条件抑制运动性具有抗性。这些突变被定位到42分钟处，这是染色体上许多鞭毛基因所在的区域。我们还研究了预先存在的gyrA突变的影响，该突变使得在萘啶酸存在的情况下能够形成鞭毛。
