Phototaxis mutants of Chlamydomonas reinhardtii.

A group of 31 mutants of Chlamydomonas reinhardtii with altered phototactic responses was isolated. Growth of all the mutants was normal and, with one exception, they had no gross morphological aberrations. One mutant produced supernumerary flagella. The mutants can be subdivided into five phenotypic classes on the basis of their abnormal responses: slow positive, no reponse, slow negative, fast negative, and variable. Random motility of the mutants was also characterized. About half were indistinguishable from wild type; the others exhibited "jerky" or "twirly" movements instead of relatively straight paths. These mutants can now be used for genetic and biochemical studies of the cellular basis of phototaxis.