拉各斯蝙蝠病毒的系统发育：狂犬病病毒分类面临的挑战。

Phylogeny of Lagos bat virus: challenges for lyssavirus taxonomy.

作者信息

Markotter W, Kuzmin I, Rupprecht C E, Nel L H

机构信息

Department of Microbiology and Plant Pathology, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, South Africa.

出版信息

Virus Res. 2008 Jul;135(1):10-21. doi: 10.1016/j.virusres.2008.02.001. Epub 2008 Mar 24.

DOI:10.1016/j.virusres.2008.02.001

PMID:18359532

Abstract

Lagos bat virus (LBV) belongs to genotype 2 of the Lyssavirus genus. The complete nucleoprotein (N), phosphoprotein (P), matrixprotein (M) and glycoprotein (G) genes of 13 LBV isolates were sequenced and phylogenetically compared with other lyssavirus representatives. The results identified three different lineages of LBV. One of these lineages demonstrated sufficient sequence diversity to be considered a new lyssavirus genotype (Dakar bat lyssavirus). The suggested quantitative separation of lyssavirus genotypes using the N, P, M and G genes was also investigated using P-distances matrixes. Results indicated that the current criteria should be revised since overlaps between intergenotypic and intragenotypic variation occur.

摘要

拉各斯蝙蝠病毒（LBV）属于狂犬病毒属的2型。对13株LBV分离株的完整核蛋白（N）、磷蛋白（P）、基质蛋白（M）和糖蛋白（G）基因进行了测序，并与其他狂犬病毒代表株进行了系统发育比较。结果确定了LBV的三个不同谱系。其中一个谱系表现出足够的序列多样性，可被视为一种新的狂犬病毒基因型（达喀尔蝙蝠狂犬病毒）。还使用P距离矩阵研究了使用N、P、M和G基因对狂犬病毒基因型进行定量分离的建议。结果表明，由于基因型间和基因型内变异存在重叠，目前的标准应予以修订。