Sato N, Kashima K, Shimizu H, Uehara Y, Shimomura Y, Mori M
First Department of Internal Medicine, Gunma University School of Medicine, Japan.
Life Sci. 1993;52(18):1481-6. doi: 10.1016/0024-3205(93)90109-g.
We studied the influence of graded degrees of hypertonic glucose or sucrose on the generation of oxygen-derived free radicals by rat neutrophils. Hypertonic glucose and sucrose exerted dose- and time-dependent inhibition of chemiluminescence amplified by luciferin analog (CLA-DCL) and luminol (L-DCL) in response to fMLP. Hypertonic glucose was more effective to this chemiluminescence inhibition than hypertonic sucrose was. This inhibition of hypertonicity was more effective in CLA-DCL than in L-DCL. Although the production of superoxide anion measured by the reduction of ferricytochrome c was more inhibited by hypertonic glucose than by hypertonic sucrose, the myeloperoxidase activity was not affected by either glucose or sucrose hyperosmolarity. These data suggest that hyperosmotic state by itself and an additional direct glucose-toxicity may contribute to the impaired neutrophil function in the diabetic state.
我们研究了不同浓度的高渗葡萄糖或蔗糖对大鼠中性粒细胞氧衍生自由基生成的影响。高渗葡萄糖和蔗糖对荧光素类似物（CLA - DCL）和鲁米诺（L - DCL）放大的、对fMLP反应的化学发光具有剂量和时间依赖性抑制作用。高渗葡萄糖对这种化学发光抑制作用比高渗蔗糖更有效。这种高渗性的抑制在CLA - DCL中比在L - DCL中更有效。尽管通过高铁细胞色素c还原法测定的超氧阴离子产生受到高渗葡萄糖的抑制比高渗蔗糖更明显，但髓过氧化物酶活性不受葡萄糖或蔗糖高渗状态的影响。这些数据表明，高渗状态本身以及额外的直接葡萄糖毒性可能导致糖尿病状态下中性粒细胞功能受损。
