Sato N, Shimizu H
First Department of Internal Medicine, Gunma University School of Medicine, Japan.
Diabetes. 1993 Mar;42(3):470-3. doi: 10.2337/diab.42.3.470.
To evaluate whether G-CSF improves an impaired production of oxygen-derived free radicals in diabetic neutrophils, we studied the effect of G-CSF on chemiluminescence amplified by a luciferin analog (CLA-DCL) and luminol (L-DCL) in response to fMLP in neutrophils from STZ-induced diabetic rats. Both CLA-DCL and L-DCL in diabetic neutrophils were significantly reduced, and L-DCL was more sensitive to this inhibition than CLA-DCL. G-CSF did not change the basal chemiluminescence in either control or diabetic neutrophils, but it apparently primed CLA-DCL and L-DCL. Although, in diabetic neutrophils, the priming effect of G-CSF to both CLA-DCL and L-DCL was less compared with that in control neutrophils, L-DCL was more sensitive than CLA-DCL to this priming effect. Because bacterial infection is still an important cause of morbidity and mortality in diabetic patients, these data suggest that G-CSF may be useful as a drug to prevent the aggravation of bacterial infection in diabetic patients.
为了评估粒细胞集落刺激因子（G-CSF）是否能改善糖尿病中性粒细胞中氧衍生自由基生成受损的情况，我们研究了G-CSF对链脲佐菌素诱导的糖尿病大鼠中性粒细胞中，由荧光素类似物（CLA-DCL）和鲁米诺（L-DCL）放大的化学发光响应fMLP的影响。糖尿病中性粒细胞中的CLA-DCL和L-DCL均显著降低，且L-DCL对这种抑制作用比CLA-DCL更敏感。G-CSF在对照或糖尿病中性粒细胞中均未改变基础化学发光，但它明显增强了CLA-DCL和L-DCL。虽然在糖尿病中性粒细胞中，G-CSF对CLA-DCL和L-DCL的增强作用与对照中性粒细胞相比有所减弱，但L-DCL对这种增强作用比CLA-DCL更敏感。由于细菌感染仍是糖尿病患者发病和死亡的重要原因，这些数据表明G-CSF可能作为一种药物用于预防糖尿病患者细菌感染的加重。
