Waumans L, Claeys H, Verhaert H, Mertens W, Vermylen C
Belgian Red Cross Blood Transfusion Centre, Leuven.
Vox Sang. 1993;64(3):145-9. doi: 10.1111/j.1423-0410.1993.tb05152.x.
A combination of different enzyme immunoassays (EIAs) was used for the serological confirmation of sera that were positive in a hepatitis C virus (HCV) second-generation screening EIA. Different reaction patterns were related with the probability of the HCV-carrier state as determined by polymerase chain reaction (PCR). Five hundred and eight sera of volunteer blood donors were send for confirmation and at first reexamined with both Abbott and Ortho second-generation screening EIA. A group of 195 sera, positive in both assays, was further evaluated by the Abbott Supplemental Assay, the Monolisa anti-HCV and an EIA with only the amino terminal part of the nucleocapsid protein as antigen. In addition PCR on the 5'-noncoding region of the viral genome was performed. We observed that 75 of the 78 PCR-positive sera were found in a group of 89 sera that were strongly positive in the four EIAs used. Moreover all but 1 PCR-positive sera were reactive against the nucleocapsid protein of the virus. Hence we concluded that a genuine antibody response to the nucleocapsid protein is highly suggestive for the HCV-carrier state.
采用不同的酶免疫测定法（EIA）组合对丙型肝炎病毒（HCV）第二代筛查EIA呈阳性的血清进行血清学确认。不同的反应模式与通过聚合酶链反应（PCR）确定的HCV携带状态的可能性相关。将508份无偿献血者的血清送去进行确认，首先用雅培和奥索第二代筛查EIA进行复查。对在两种检测中均呈阳性的195份血清，进一步用雅培补充检测、莫诺丽莎抗HCV检测以及一种以核衣壳蛋白仅氨基末端部分作为抗原的EIA进行评估。此外，还对病毒基因组的5'-非编码区进行了PCR检测。我们观察到，在使用的四种EIA中呈强阳性的89份血清中，发现了78份PCR阳性血清中的75份。而且，除1份PCR阳性血清外，所有血清均对病毒的核衣壳蛋白呈反应性。因此我们得出结论，对核衣壳蛋白的真正抗体反应高度提示HCV携带状态。
