De Beenhouwer H, Verhaert H, Claeys H, Vermylen C
Belgian Red Cross Blood Transfusion Centre, Leuven.
Vox Sang. 1992;63(3):198-203. doi: 10.1111/j.1423-0410.1992.tb05100.x.
In a series of 385 sera obtained from volunteer blood donors positive for the first-generation hepatitis C virus assay (Ortho), the viral genome was detected by polymerase chain reaction (PCR) in 89 sera (23%). Most PCR-positive sera were found positive with the c100-3 neutralisation assay (Abbott) and by two second-generation enzyme immunoassays (Abbott, Ortho). However overall specificity of these assays was rather low. By immunoblotting (Innogenetics and Chiron/Ortho) the specificity could be considerably improved and the best correlation with carrier state was obtained when analysing the results for lane-specific reaction: all 89 viral carriers and only 9 other donors had antibodies against structural 'core' epitopes. From the present data we can conclude that in screening a volunteer blood donor population the confirmation of antibodies against 'core' epitopes by immunoblotting is strongly associated with viral carriage.
在一组从第一代丙型肝炎病毒检测（Ortho）呈阳性的志愿献血者中获得的385份血清中，通过聚合酶链反应（PCR）在89份血清（23%）中检测到病毒基因组。大多数PCR阳性血清通过c100 - 3中和试验（雅培）和两种第二代酶免疫测定法（雅培、Ortho）检测为阳性。然而，这些检测方法的总体特异性相当低。通过免疫印迹法（Innogenetics和Chiron/Ortho），特异性可得到显著提高，并且在分析泳道特异性反应结果时与携带状态的相关性最佳：所有89名病毒携带者以及仅9名其他献血者具有针对结构“核心”表位的抗体。根据目前的数据我们可以得出结论，在筛查志愿献血者群体时，通过免疫印迹法确认针对“核心”表位的抗体与病毒携带密切相关。
