Skaug K, Li H, Jonassen T O, Larsen J, Figenschau K J
Department of Microbiology, Ullevaal Hospital, Oslo, Norway.
Vox Sang. 1993;64(4):215-9. doi: 10.1111/j.1423-0410.1993.tb03058.x.
Seven of 24 blood donors positive in Ortho's first-generation antibody to hepatitis C virus (anti-HCV) test (EIA-1) were also positive in Ortho's second-generation test (EIA-2). All 7 had at least two anti-HCV positive recipients, whereas only 1 of the 17 EIA-2-negative donors had an anti-HCV-positive recipient. This recipient was a multitransfused patient with von Willebrand's disease. Five of the 7 EIA-2-positive donors had detectable HCV RNA. We traced and tested 38 of the still living blood recipients from the 7 EIA-2-positive donors. Twenty-eight of these were EIA-2 positive and 22 were HCV-PCR positive. One patient with Waldenstroem's hypergammaglobulinemia was EIA-2 negative but HCV-PCR positive. All the EIA-2-positive sera showed reactivity in Ortho's recombinant immunoblot assay (RIBA-2), but 5 of the 28 recipients and 1 of the donors reacted with only one band (RIBA-2 indeterminate). Among 32 recipients who probably had received EIA-2-positive blood, 29 (91%) had markers of an HCV infection. Twenty-two (75%) of the HCV-infected recipients had detectable HCV RNA more than 6 months after transfusion and hence were chronic HCV carriers.
在 Ortho 第一代丙型肝炎病毒抗体（抗 - HCV）检测（酶免疫测定 - 1，EIA - 1）中呈阳性的 24 名献血者中，有 7 人在 Ortho 第二代检测（EIA - 2）中也呈阳性。这 7 名献血者的所有受血者中至少有两名抗 - HCV 呈阳性，而在 17 名 EIA - 2 阴性的献血者中，只有 1 人的受血者抗 - HCV 呈阳性。该受血者是一名患有血管性血友病的多次输血患者。7 名 EIA - 2 阳性的献血者中有 5 人可检测到 HCV RNA。我们追踪并检测了来自这 7 名 EIA - 2 阳性献血者的 38 名仍在世的受血者。其中 28 人 EIA - 2 呈阳性，22 人 HCV - PCR 呈阳性。一名患有华氏巨球蛋白血症的患者 EIA - 2 呈阴性但 HCV - PCR 呈阳性。所有 EIA - 2 阳性血清在 Ortho 的重组免疫印迹测定（RIBA - 2）中均显示出反应性，但 28 名受血者中的 5 人以及 1 名献血者仅与一条带发生反应（RIBA - 2 不确定）。在可能接受了 EIA - 2 阳性血液的 32 名受血者中，29 人（91%）有 HCV 感染标志物。22 名（75%）感染 HCV 的受血者在输血后 6 个月以上可检测到 HCV RNA，因此是慢性 HCV 携带者。
