多金属氧酸盐的体外抗病毒活性。PM - 104（(NH4)12H2(Eu4(MoO4)(H2O)16(Mo7O24)4).13H2O）对1型人类免疫缺陷病毒的抑制作用机制。

In vitro antiviral activity of polyoxomolybdates. Mechanism of inhibitory effect of PM-104 (NH4)12H2(Eu4(MoO4)(H2O)16(Mo7O24)4).13H2O on human immunodeficiency virus type 1.

作者信息

Inouye Y, Tokutake Y, Yoshida T, Seto Y, Hujita H, Dan K, Yamamoto A, Nishiya S, Yamase T, Nakamura S

机构信息

Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, Japan.

出版信息

Antiviral Res. 1993 Apr;20(4):317-31. doi: 10.1016/0166-3542(93)90075-t.

DOI:10.1016/0166-3542(93)90075-t

PMID:8387260

Abstract

A screening for inhibitors of human immunodeficiency virus type 1 (HIV-1) among various types of isopolyoxomolybdates and heteropolyoxomolybdates was carried out by using an in vitro assay system measuring the cytopathogenicity of HIV-1 in CD4+ human MT-4 cells. A novel heteropolyoxomolybdate named PM-104 with the chemical formula (NH4)12H2(Eu4(MoO4)(H2O)16(Mo7O24)4).13H2O was found to be associated with potent anti-HIV-1 activity. PM-104 interferes with virus infection at a very early step such as adsorption and/or penetration into the cells. In addition to the cytopathic effect of HIV-1 on MT-4 cells, syncytium formation between mock-infected MOLT-4 cells and MOLT-4 cells chronically infected with either HIV-1 or HIV-2 is suppressed by PM-104. PM-104 also blocks the replication of herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2). The antiviral properties of PM-104 could be attributed to the combined effect of europium atoms and its peculiar three-dimensional anion structure.

摘要

通过使用一种体外检测系统，该系统可测量HIV-1在CD4 + 人MT-4细胞中的细胞致病性，对各种类型的同多氧钼酸盐和杂多氧钼酸盐进行了人类免疫缺陷病毒1型（HIV-1）抑制剂的筛选。发现一种化学式为(NH4)12H2(Eu4(MoO4)(H2O)16(Mo7O24)4).13H2O的新型杂多氧钼酸盐PM-104具有强大的抗HIV-1活性。PM-104在病毒感染的非常早期阶段，如吸附和/或侵入细胞时发挥干扰作用。除了HIV-1对MT-4细胞的细胞病变效应外，PM-104还可抑制未感染的MOLT-4细胞与长期感染HIV-1或HIV-2的MOLT-4细胞之间的多核巨细胞形成。PM-104还可阻断单纯疱疹病毒1型（HSV-1）和2型（HSV-2）的复制。PM-104的抗病毒特性可能归因于铕原子及其独特的三维阴离子结构的综合作用。