Izzo R S, Witkon K, Chen A I, Hadjiyane C, Weinstein M I, Pellecchia C
Dept. of Medicine, Nassau County Medical Center, East Meadow, New York.
Scand J Gastroenterol. 1993 Apr;28(4):296-300. doi: 10.3109/00365529309090244.
We considered the role of two neutrophil chemotactic agents (interleukin-8 and leukotriene B4) and of myeloperoxidase (a neutrophil-associated enzyme) in the pathologic condition of Crohn's disease (CD). Serial biopsy samples were taken at different sites in the colon, washed in 0.02 M phosphate-saline buffer, homogenized, and then sonicated. Interleukin-8 levels were significantly increased throughout the colonic mucosa (> 300 pg/mg protein) in patients with CD compared with control groups (< 40 pg/mg protein) (p < or = 0.01). A two- to six-fold increase in leukotriene B4 was also found in CD, whereas mucosal levels of myeloperoxidase were unchanged compared with control subjects. This study demonstrates that interleukin-8 and leukotriene B4 may have an immunologic role in the pathologic condition of CD.
我们研究了两种中性粒细胞趋化因子（白细胞介素-8和白三烯B4）以及髓过氧化物酶（一种与中性粒细胞相关的酶）在克罗恩病（CD）病理状况中的作用。在结肠的不同部位采集系列活检样本，用0.02M磷酸盐缓冲盐水冲洗，匀浆，然后超声处理。与对照组（<40 pg/mg蛋白质）相比，CD患者整个结肠黏膜中的白细胞介素-8水平显著升高（>300 pg/mg蛋白质）（p≤0.01）。在CD患者中还发现白三烯B4增加了2至6倍，而与对照组相比，黏膜髓过氧化物酶水平没有变化。这项研究表明，白细胞介素-8和白三烯B4可能在CD的病理状况中发挥免疫作用。
