Izzo R S, Witkon K, Chen A I, Hadjiyane C, Weinstein M I, Pellecchia C
Department of Medicine, Nassau County Medical Center, East Meadow, New York.
Am J Gastroenterol. 1992 Oct;87(10):1447-52.
In this study, mediators of inflammation were characterized in colonic and terminal ileum mucosa from subjects with ulcerative colitis. We considered the role of two different chemotactic factors (interleukin-8 and leukotriene B4) and of myeloperoxidase in the pathology of inflammatory bowel disease. Serial biopsy specimens were taken at different sites, washed in 0.02 M phosphate/saline buffer, homogenized, and then sonically disrupted. In both the proximal and distal regions of the colonic mucosa of ulcerative colitis patients, there was a more than 10-fold increase in interleukin-8 levels over that in control subjects (> 300 pg/mg protein vs. 30 pg/mg protein in controls, p < or = 0.01). However, terminal ileum levels of interleukin-8 were the same in ulcerative colitis and control groups (150 pg/mg protein). There was also a 3- to 5-fold increase in leukotriene B4 levels and a several-fold increase in myeloperoxidase levels throughout the colonic mucosa in patients with ulcerative colitis. This study demonstrates that 1) interleukin-8 may have an immunoregulatory role in the pathogenesis of inflammatory bowel disease, and 2) interleukin-8, myeloperoxidase, and leukotriene B4 may be useful markers for the biochemical identification of inflammatory bowel disease.
在本研究中，对溃疡性结肠炎患者的结肠和回肠末端黏膜中的炎症介质进行了特征分析。我们探讨了两种不同趋化因子（白细胞介素-8和白三烯B4）以及髓过氧化物酶在炎症性肠病病理过程中的作用。在不同部位采集系列活检标本，用0.02M磷酸盐/生理盐水缓冲液冲洗，匀浆，然后超声破碎。在溃疡性结肠炎患者结肠黏膜的近端和远端区域，白细胞介素-8水平均比对照受试者增加了10倍以上（>300 pg/mg蛋白质，而对照为30 pg/mg蛋白质，p≤0.01）。然而，溃疡性结肠炎组和对照组的回肠末端白细胞介素-8水平相同（150 pg/mg蛋白质）。溃疡性结肠炎患者整个结肠黏膜中的白三烯B4水平也增加了3至5倍，髓过氧化物酶水平增加了数倍。本研究表明：1）白细胞介素-8可能在炎症性肠病的发病机制中具有免疫调节作用；2）白细胞介素-8、髓过氧化物酶和白三烯B4可能是用于炎症性肠病生化鉴定的有用标志物。
