Goto S, Nagahiro S, Korematsu K, Ushio Y
Department of Neurosurgery, Kumamoto University Medical School, Japan.
Acta Neuropathol. 1993;85(5):515-20. doi: 10.1007/BF00230491.
A topographical and cellular immunohistochemical analysis was performed on the striatonigral system of rats with unilateral, reversible middle cerebral artery (MCA) occlusion. Antibodies to calcineurin (CaN), parvalbumin (PV), choline acetyltransferase (ChAT) and glial fibrillary acidic protein (GFAP) were used in this study. Sixty days after the operation, the ipsilateral striatum showed a characteristic cell type-specific injury in the dorsolateral part of the nucleus (i.e., non-limbic striatum): a marked reduction in the number of medium-sized spinous neurons expressing CaN immunoreactivity and a selective sparing of PV- and ChAT-positive interneurons. There was also a marked depletion of striatonigral afferents visualized by CaN immunostaining in the lateral portion of the substantia nigra pars reticulata, which is considered to be implicated with motor function. In addition, it was noted that such striatonigral involvement was accompanied by marked gliosis showing strong GFAP immunolabeling. The present data suggest that rats with reversible MCA occlusion can be a useful animal model for studying cell type-specific ischemic injury and subdivisional involvement of the striatonigral pathway as a part of the cortico-subcortical loop subserving motor function.
对单侧、可逆性大脑中动脉（MCA）闭塞的大鼠的纹状体黑质系统进行了拓扑学和细胞免疫组织化学分析。本研究使用了针对钙调神经磷酸酶（CaN）、小白蛋白（PV）、胆碱乙酰转移酶（ChAT）和胶质纤维酸性蛋白（GFAP）的抗体。术后60天，同侧纹状体在核的背外侧部分（即非边缘纹状体）显示出特征性的细胞类型特异性损伤：表达CaN免疫反应性的中型棘状神经元数量显著减少，而PV和ChAT阳性中间神经元选择性保留。通过CaN免疫染色在黑质网状部外侧部分可视化的纹状体黑质传入纤维也明显减少，这被认为与运动功能有关。此外，还注意到这种纹状体黑质受累伴有明显的胶质增生，表现为强烈的GFAP免疫标记。目前的数据表明，可逆性MCA闭塞的大鼠可以作为一种有用的动物模型，用于研究细胞类型特异性缺血性损伤以及作为服务于运动功能的皮质-皮质下环路一部分的纹状体黑质通路的分区受累情况。
