Miller J K, Laycock K A, Nash M M, Pepose J S
Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St. Louis, MO 63110.
Invest Ophthalmol Vis Sci. 1993 Jun;34(7):2282-90.
The authors investigated the progressive changes in the distribution of corneal Langerhans cells (LC) after reactivation of latent herpes simplex virus type 1 (HSV-1) in mice.
After corneal inoculation of National Institutes of Health inbred mice with HSV-1 and the establishment of latency, viral reactivation was induced by irradiating the ocular surface with 250 mJ/cm2 of ultraviolet B (UV-B) light.
Subsequent viral replication in the cornea was followed by the migration of the LC toward the paracentral and central corneal epithelium. These areas are normally devoid of LC. The number of LC in the paracentral and central regions of the eye reached a peak at day 14 post-UV-B irradiation. After UV-B irradiation of mice latently infected with HSV-1, the development of corneal stromal opacification and neovascularization closely followed the migration of LC toward the central cornea and paralleled the influx of T-cells into the corneal stroma. This pattern was not observed in irradiated uninfected mice.
LC migrate centrally in the corneal epithelium after viral reactivation. There is a direct correlation between the number of LC in the cornea and the degree of persistent stromal opacification.
作者研究了小鼠潜伏的单纯疱疹病毒1型（HSV-1）重新激活后角膜朗格汉斯细胞（LC）分布的渐进性变化。
用HSV-1接种美国国立卫生研究院近交系小鼠角膜并建立潜伏感染后，通过用250 mJ/cm2的紫外线B（UV-B）照射眼表诱导病毒重新激活。
随后角膜中的病毒复制伴随着LC向角膜旁中央和中央上皮迁移。这些区域通常没有LC。眼的旁中央和中央区域的LC数量在UV-B照射后第14天达到峰值。在潜伏感染HSV-1的小鼠接受UV-B照射后，角膜基质混浊和新生血管形成的发展紧密跟随LC向中央角膜的迁移，并与T细胞流入角膜基质平行。在接受照射的未感染小鼠中未观察到这种模式。
病毒重新激活后，LC在角膜上皮中向中央迁移。角膜中LC的数量与持续性基质混浊的程度之间存在直接相关性。
