Xiao H, Crombie R, Dong Z, Onrust R, O'Donnell M
Microbiology Department, Hearst Research Foundation, Cornell University Medical College, New York, New York 10021.
J Biol Chem. 1993 Jun 5;268(16):11773-8.
Genes encoding the chi and psi accessory proteins of the DNA polymerase III holoenzyme replicase of Escherichia coli have been identified, sequenced, and used to express and purify both chi and psi in quantity. The holC gene encoding chi is located between the xerB and valS genes at 96.5 min on the chromosome; it encodes a 147-amino acid protein of 16.6 kDa. holD encoding psi lies upstream of rimI at 99.3 min and encodes a 137-amino acid protein of 15.2 kDa. The genes have been cloned into expression vectors, and both chi and psi have been purified in quantity. The accompanying report characterizes the function and physical interactions of chi and psi with other holenzyme subunits (Xiao, H., Dong, Z., and O'Donnell, M. (1993) J. Biol. Chem. 268, 11779-11784).
编码大肠杆菌DNA聚合酶III全酶复制酶的chi和psi辅助蛋白的基因已被鉴定、测序，并用于大量表达和纯化chi和psi。编码chi的holC基因位于染色体上96.5分钟处的xerB和valS基因之间；它编码一个16.6 kDa的147个氨基酸的蛋白质。编码psi的holD位于99.3分钟处的rimI上游，编码一个15.2 kDa的137个氨基酸的蛋白质。这些基因已被克隆到表达载体中，并且chi和psi都已大量纯化。随附的报告描述了chi和psi与其他全酶亚基的功能及物理相互作用（Xiao, H., Dong, Z., and O'Donnell, M. (1993) J. Biol. Chem. 268, 11779 - 11784）。
