Velasco J, Palacio V, Vazquez S, Mosquera C, Sampedro A
Facultad de Medicina, Universidad de Oviedo, Anatomia Patologica, Hospital San Agustin, Aviles, Spain.
Sex Transm Dis. 1993 May-Jun;20(3):147-51. doi: 10.1097/00007435-199305000-00006.
During the years 1990 to 1991, smears taken from the anal canal of 45 randomly selected homosexual males were studied. Several cytological criteria were used to study infection by human papillomavirus (HPV). The cytological study and an anoscopy was performed and a swab was taken for HPV-DNA hybridization, using labelled RNA probes for HPV-DNA: 6,11,16,18,31,33, and 35. In our study we observed the existence of a high proportion (9.5%) of inadequate smears that did not contain glandular cells and/or metaplasia. A sensitivity of 78% and a specificity of 72% was obtained. Sensitivity and specificity were 68% and 91%, respectively, when these criteria were restricted to the presence of koilocytes and/or dyskeratocytes. The high rate of occult infections obtained in our study lead us to suggest the simultaneous use of cytology, molecular hybridization, and anoscopy for suitable care of those patients with a high risk of contracting HPV infections of the anal canal.
在1990年至1991年期间，对45名随机选取的男同性恋者肛管采集的涂片进行了研究。采用了几种细胞学标准来研究人乳头瘤病毒（HPV）感染情况。进行了细胞学研究和肛门镜检查，并采集拭子用于HPV-DNA杂交，使用针对HPV-DNA的标记RNA探针检测6、11、16、18、31、33和35型。在我们的研究中，观察到存在高比例（9.5%）的不合格涂片，这些涂片不含腺细胞和/或化生细胞。获得的灵敏度为78%，特异性为72%。当这些标准仅限于存在空泡细胞和/或异常角化细胞时，灵敏度和特异性分别为68%和91%。我们研究中获得的隐匿性感染高发生率促使我们建议同时使用细胞学、分子杂交和肛门镜检查，以便对那些有肛管感染HPV高风险的患者进行适当护理。
