通过聚合酶链反应检测牛免疫缺陷样病毒。
Detection of bovine immunodeficiency-like virus by the polymerase chain reaction.
作者信息
Nadin-Davis S A, Chang S C, Smith H, Jacobs R M
机构信息
Agriculture Canada, Animal Diseases Research Institute, NEPEAN, Ontario.
出版信息
J Virol Methods. 1993 May;42(2-3):323-36. doi: 10.1016/0166-0934(93)90043-q.
This report describes a test for the bovine lentivirus, bovine immunodeficiency-like virus, in which polymerase chain reaction (PCR) technology is employed. Two pairs of oligonucleotide primers directed to sequences within the coding regions of the gag and pol genes generated the expected PCR products from molecular BIV clones and from DNA of BIV-infected cell cultures but not from DNA of uninfected cultures. Data indicating the specificity and sensitivity of these PCRs for BIV detection and the potential utility of this technology for diagnostic applications are presented.
本报告描述了一种针对牛慢病毒（牛免疫缺陷样病毒）的检测方法，该方法采用了聚合酶链反应（PCR）技术。两对针对gag和pol基因编码区域内序列的寡核苷酸引物，从分子BIV克隆以及BIV感染的细胞培养物DNA中扩增出了预期的PCR产物，但未从未感染培养物的DNA中扩增出产物。文中给出了这些PCR检测BIV的特异性和灵敏度数据，以及该技术在诊断应用中的潜在效用。
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