Saltzstein D R, Orihuela E, Kocurek J N, Payne D A, Chan T S, Tyring S K
Division of Urology, University of Texas Medical Branch, Galveston 77555-0540.
Anticancer Res. 1993 Mar-Apr;13(2):423-5.
In contrast to cervical and penile carcinoma, in situ hybridization techniques have not been able to demonstrate an association of HPV with transitional cell carcinoma (TCC) of the bladder. The introduction of the polymerase chain reaction (PCR) in the mid 1980s has significantly increased the ability to detect small quantities of viral DNA over conventional methods. Thus, we designed a study to determine if the PCR technique was able to demonstrate the presence of HPV DNA in TCC specimens. The study involved both consensus primers directed toward the E1 and L1 open reading frames of the HPV viral DNA, specific for HPV 6, 11, 16, 18, 31, 33. Thirty-three TCC specimens were studied (Fresh: 8, paraffin embedded: 25). Seven were Grade I, nine Grade II, seventeen Grade III; thirteen were superficial (Stages 0 and A) and twenty were invasive or metastatic (Stages B or Higher). None of the patients had known evidence of clinical HPV infection. In each experiment, the CaSki cell line was used for a positive control. In addition, the results of the PCR reactions were confirmed by Southern blot hybridization. Neither the PCR by direct ethidium bromide viewing, nor the Southern blot technique detected HPV DNA in any of the TCC specimens. This was in contrast to our controls, which were positive by both techniques. Although it is possible that there is a link between HPV and TCC, our results suggest that there is no such association among the HPV types tested.
与宫颈癌和阴茎癌不同，原位杂交技术未能证实人乳头瘤病毒（HPV）与膀胱移行细胞癌（TCC）有关联。20世纪80年代中期聚合酶链反应（PCR）的引入，相较于传统方法，显著提高了检测少量病毒DNA的能力。因此，我们设计了一项研究，以确定PCR技术能否在TCC标本中检测到HPV DNA。该研究使用了针对HPV病毒DNA的E1和L1开放阅读框的共有引物，这些引物对HPV 6、11、16、18、31、33具有特异性。共研究了33个TCC标本（新鲜标本：8个，石蜡包埋标本：25个）。其中7个为I级，9个为II级，17个为III级；13个为浅表性（0期和A期），20个为浸润性或转移性（B期或更高分期）。所有患者均无临床HPV感染的已知证据。在每个实验中，CaSki细胞系用作阳性对照。此外，PCR反应结果通过Southern印迹杂交进行确认。无论是通过直接溴化乙锭观察的PCR，还是Southern印迹技术，均未在任何TCC标本中检测到HPV DNA。这与我们的对照相反，我们的对照在两种技术下均为阳性。尽管HPV与TCC之间可能存在联系，但我们的结果表明，在所检测的HPV类型中不存在这种关联。
