Claas E C, Melchers W J, van der Linden H C, Lindeman J, Quint W G
Diagnostic Centre, SSDZ, Department of Pathology, Delft, The Netherlands.
Am J Pathol. 1989 Oct;135(4):703-9.
The polymerase chain reaction (PCR) is used for human papillomavirus (HPV) detection in paraffin-embedded tissue. The specificity of the reaction is unaffected by the method of fixation used before embedding into paraffin. Five HPV 16, 18, 31, and 33 DNA in situ hybridization (DISH)-negative cervical carcinomas were subjected to the PCR. In two patients, HPV 16 DNA could be detected in the cervical squamous cell carcinomas and also in their lymph node metastases. One patient with an adeno-carcinoma of the cervix was found positive for HPV-18. A lymph node of this patient was HPV 18 positive as well. In the tumors of the remaining two patients, no HPV 16, 18, or 33 DNA was detected by the PCR. Both negative patients had cervical squamous cell carcinomas. One had a bladder metastasis, whereas the other had a lymph node metastasis and an additional distant metastasis in the lung. HPV DNA positivity in cervical carcinomas correlated with HPV prevalence in the metastases. This relationship can be of use for diagnostic purposes in the pathologic analysis of metastases.
聚合酶链反应（PCR）用于检测石蜡包埋组织中的人乳头瘤病毒（HPV）。该反应的特异性不受石蜡包埋前所用固定方法的影响。对5例原位杂交（DISH）检测人乳头瘤病毒16、18、31和33型DNA均为阴性的宫颈癌进行PCR检测。在2例患者中，在宫颈鳞状细胞癌及其淋巴结转移灶中均检测到HPV 16 DNA。1例宫颈腺癌患者HPV - 18检测呈阳性，该患者的一个淋巴结HPV 18也呈阳性。在其余2例患者的肿瘤中，PCR未检测到HPV 16、18或33型DNA。这2例阴性患者均为宫颈鳞状细胞癌，其中1例有膀胱转移，另1例有淋巴结转移且肺部还有远处转移。宫颈癌中的HPV DNA阳性与转移灶中的HPV流行率相关。这种关系在转移灶的病理分析中可用于诊断目的。
