Smetana Z, Keller T, Leventon-Kriss S, Huszar M, Lindner A, Mitrani-Rosenbaum S, Mendelson E, Smetana S
Central Virology Laboratory, Chaim Sheba Medical Center, Tel-Hashomer, Ramat Gan, Israel.
Cell Mol Biol (Noisy-le-grand). 1995 Dec;41(8):1017-23.
The possible causal association of human papilloma virus (HPV) with transitional cell carcinoma (TCC) of the urinary bladder in Israeli Jewish patients was assessed. One hundred and ten histopathological TCC sections were examined by peroxidase anti-peroxidase (PAP) method. HPV capsid antigen was demonstrated in 19 out of 110 cases (17.3%). HPV-DNA sequences, determined by in situ DNA-DNA hybridization at high stringency wash were present in 24 cases (21.8%): 16(14.5%) cases proved to be HPV6/11 and 8 (7.3%) were HPV 16/18 positive. Four (3.6%) of the HPV 6/11 positive specimens cross hybridized with HPV 31/33/35 at low stringency conditions. Sixteen samples known to be positive by in situ hybridization were reconfirmed by polymerase chain reaction (PCR). When the PCR was performed on the 43 negative cases, an additional 4(9.3%) HPV positive cases were revealed: two proved to be HPV 6/11 and two HPV 16/18. Comparison of the different methods for HPV detection in 59 TCC histopathological samples, showed good correlation; an overall positivity of 33.9% by PCR, 27.1% by in situ hybridization and 25.4% by PAP was observed. Forty one samples from nontumoral material of the bladder or post mortem specimens served as controls and 4.8% HPV DNA was present in only two cases: one HPV 6/11 and one 16/18. Hence, HPV in TCC of the bladder is detected at a relatively high frequency and might be involved in the pathogenesis of this tumor among Jewish population in Israel.
评估了人类乳头瘤病毒（HPV）与以色列犹太患者膀胱移行细胞癌（TCC）之间可能的因果关联。采用过氧化物酶抗过氧化物酶（PAP）法检查了110份TCC组织病理学切片。110例中有19例（17.3%）显示HPV衣壳抗原。通过高严谨度洗涤的原位DNA-DNA杂交确定的HPV-DNA序列存在于24例（21.8%）中：16例（14.5%）为HPV6/11阳性，8例（7.3%）为HPV 16/18阳性。4例（3.6%）HPV 6/11阳性标本在低严谨度条件下与HPV 31/33/35交叉杂交。通过聚合酶链反应（PCR）再次确认了16份已知原位杂交阳性的样本。对43例阴性病例进行PCR检测时，又发现4例（9.3%）HPV阳性病例：2例为HPV 6/11，2例为HPV 16/18。对59份TCC组织病理学样本中不同HPV检测方法的比较显示出良好的相关性；PCR总体阳性率为33.9%，原位杂交为27.1%，PAP为25.4%。来自膀胱非肿瘤组织或尸检标本的41份样本用作对照，仅2例（4.8%）存在HPV DNA：1例为HPV 6/11，1例为16/18。因此，在以色列犹太人群中，膀胱TCC中HPV的检测频率相对较高，可能参与了该肿瘤的发病机制。
