Danciger M, Farber D B, Kozak C A
Jules Stein Eye Institute, UCLA School of Medicine.
Genomics. 1993 May;16(2):361-5. doi: 10.1006/geno.1993.1198.
GABAA receptors are a heterogeneous group of heterooligomeric ligand-gated anion channels that represent the main means of synaptic inhibition in neuronal tissue. At least 14 distinct GABAA subunit proteins representing 5 different classes have been identified. Using rat brain cDNAs for the GABAA receptor beta 1-, alpha 2-, and alpha 4-subunits, we have assigned the corresponding murine genes Gabrb-1, Gabra-2, and Gabra-4 to specific chromosomes with somatic cell hybrids, and we have positioned these genes by analysis of the progeny of three genetic crosses. Gabrb-1 and Gabra-2 are tightly linked on mouse Chromosome 5 between the markers Pgm-1 and Kit, and Gabra-4 maps to proximal mouse Chromosome 7 in apparent proximity to the previously mapped Gabrb-3. The Gabrb-1, Gabra-2 pair of genes on Chr 5 maps near the mouse "circling mutation" pi (pirouette).
GABAA受体是一类异质性的异源寡聚配体门控阴离子通道，是神经元组织中突触抑制的主要方式。已鉴定出至少14种不同的GABAA亚基蛋白，分属5个不同类别。利用大鼠脑cDNA获得GABAA受体β1、α2和α4亚基，我们通过体细胞杂交将相应的小鼠基因Gabrb-1、Gabra-2和Gabra-4定位到特定染色体上，并通过分析三个遗传杂交的子代对这些基因进行定位。Gabrb-1和Gabra-2紧密连锁于小鼠第5号染色体上，位于标记Pgm-1和Kit之间，而Gabra-4定位于小鼠第7号染色体近端，明显靠近先前定位的Gabrb-3。第5号染色体上的Gabrb-1、Gabra-2基因对定位于小鼠“转圈突变”pi（芭蕾旋转）附近。
